597 papers
This study demonstrates that PARP16 protects against cardiac hypertrophy and heart failure by ADP-ribosylating and inhibiting the transcriptional activity of NFAT1, a mechanism whose loss leads to pathological cardiac remodeling.
This study demonstrates that inducible deletion of endothelial KLF4 in mice recapitulates the key clinical features of preeclampsia, including gestational hypertension and fetal growth restriction, thereby providing a novel model that links upstream cardiovascular risk factors to the disease's underlying mechanisms.
This study reveals that *Tetrahymena thermophila* responds to calcium-induced stress by internalizing motile cilia into ring-like structures that undergo regulated bulk degradation via autophagy, a process termed "motile ciliophagy," to recycle axonemal components for efficient ciliary regeneration and cellular homeostasis.
This study identifies the ubiquitin ligase CHFR as a critical regulator of endothelial barrier dysfunction during inflammation by mediating the K48-linked ubiquitylation and proteasomal degradation of Akt1, thereby disrupting the Ang-1/Tie2 signaling axis and promoting VE-cadherin loss.
This study employs an image-based granularity screening approach to identify specific vaccinia virus membrane proteins that induce LC3 lipidation, revealing a more directed interplay between the virus and the host autophagy pathway than previously understood.
This study demonstrates that the transcription factor ATF4 coordinates transcriptomic and structural adaptations in aging muscle by regulating the integrated stress response, which drives mitochondrial dysfunction and sarcopenia, thereby identifying the ISR pathway as a potential therapeutic target for preserving muscle function in older adults.
This study introduces fishROI, a specialized FIJI2 plugin that combines cytoplasmic staining with deep learning to provide a robust, semi-automated workflow for analyzing and visualizing the unique muscle morphometry and hyperplastic growth dynamics of teleosts.
This paper presents a streamlined protocol for rapid CRISPR-Cas9 genome editing in *Saccharomyces cerevisiae* that utilizes PCR-based protospacer installation and seamless plasmid assembly to bypass traditional restriction/ligation cloning, followed by LiAc/PEG co-transformation and PCR-based verification of edits.
The paper introduces SPEx, a cost-effective method that combines expansion microscopy with laser microdissection and mass spectrometry to achieve high-resolution subcellular proteomics, successfully identifying known and novel components of organelles like the Golgi, nucleus, and nucleoli.
This study reveals that the algal septin SEP1 physically couples chloroplast protein import and organelle division by linking TOC GTPases to the division machinery, suggesting this coordination mechanism emerged early in chloroplast evolution.
This study reveals that non-taste GPCRs exhibit functional diversity in interacting with gustducin, either activating it or forming unproductive complexes that suppress basal signaling and sequester receptors from alternative pathways, thereby establishing a novel mechanism for balancing concurrent cellular signaling.
Using in situ cryo-electron tomography on S. cerevisiae, this study reveals that cytosolic vesicle fusion contributes to phagophore expansion during later stages of autophagosome biogenesis, challenging the view that lipid transfer by the Atg2-Atg18 complex is the sole mechanism for membrane growth.
This study demonstrates that in budding yeast, the specific recruitment of the endocytic initiator Yap1802 to growing daughter cells is driven by a direct interaction with the exocytic v-SNARE Snc2 and polarized anionic phospholipids, thereby establishing a mechanistic link between polarized secretion and the initiation of clathrin-mediated endocytosis.
This multi-omics study demonstrates that ambient temperature shifts in *Chlamydomonas reinhardtii* trigger extensive molecular reorganization across transcriptomic and proteomic levels, leading to significant physiological changes in growth, motility, mating, and bacterial interactions.
This study demonstrates that the intermediate filament protein vimentin acts as a cell-scale organizer that stabilizes the global alignment and dynamics of focal adhesions, thereby converting locally stochastic adhesion events into the spatial coherence required for persistent directional cell migration.
This study demonstrates that SPIN90 selectively activates ArpC5L-containing Arp2/3 complexes to generate linear actin filaments, thereby regulating the architecture and protrusion efficiency of lamellipodia in migrating cells.
This study demonstrates that patient-derived vascularized skin organoids containing systemic sclerosis fibroblasts recapitulate key disease features, such as giant capillary formation and early fibrosis, thereby revealing the critical role of fibroblast-driven crosstalk in microvascular dysfunction and offering a novel model for drug evaluation.
This study introduces a shotgun single-cell top-down proteomics strategy that successfully profiles diverse proteoforms in individual mouse cardiomyocytes, revealing substantial cell-to-cell molecular heterogeneity and establishing a powerful tool for understanding cardiac cellular identity and physiology.
This study identifies VIA1 as an evolutionarily conserved regulator that maintains thylakoid membrane integrity under high light stress by directly binding to and functioning with the ESCRT-III-like protein VIPP1.
This study reveals that the chromatoid body orchestrates male germline mRNA regulation by integrating the piRNA, NMD, and m⁶A pathways, where the NMD endonuclease SMG6 cooperates with PIWIL1 to execute piRNA-guided degradation of m⁶A-modified transcripts.